Mediator complex structural milestones (1999-2019)

I recently wrote a review to celebrate the twentieth anniversary of the first Mediator complex EM reconstruction. This blog post is an update with clickable links (links to the papers and to the PDB/EMDB codes).

1999

First 2D EM projection of yeast Mediator complex ~ 40Å Asturias et al Science 1999

2000

3D EM low resolution (30-35 Å) of yMED, mMED and hMED Dotson et al PNAS 2000

2002

EM analysis of hMED reveals distinct conformations induced by different activators Taatjes et al Science 2002 –Näär et al Genes & Dev. 2002

3D EM reconstruction of yeast holoenzyme (Mediator + RNA pol II) ~ 35 Å Davies et al Mol Cell 2002

2005

First crystal structure of yMED subcomplex Sc 7C/21 – 3 Å (Middle module) Baumli et al JBC 2005 – PDB 1YKH

 

2006

First crystal structure of yMED subcomplex Sc 8C/18/20 – 2.7 Å (Head module) Larivière et al NSMB 2006 –PDB 2HZS

2011

Crystal structure of yMED Sc Head Module (Med6/Med8/Med11/Med17/Med18/Med20/Med22) – 4.3 Å Imasaki et al Nature 2011 –PDB 3RJ1

2012

Crystal structure of yMED Sp Head Module (Med6/Med8/Med11/Med17/Med18/Med20/Med22) – 3.4 Å Larivière et al Nature 2012 –PDB 4H63

2013

CryoEM structure of yeast CDK8 kinase module ~ 15 Å Tsai et al NSMB 2013 –EMD 5588

2014

CryoEM structure of yMED at 18 Å and EM of hMED at 30 Å Tsai et al Cell 2014 –EMD 2634 –EMD 2635

Reconstitution of a functional 15-subunit human core Mediator Cevher et al NSMB 2014

2015

3D model of full yMED by integrative modeling approach Robinson et al eLife 2015

CryoEM structure of 15-subunit core Mediator + RNA pol II 9.7 Å Plaschka et al Nature 2015 –EMD 2786 –PDB 4V1O

2016

CryoEM structure of a complete yMED-PIC at 21.9 Å Robinson et al Cell 2016 –EMD 8308

2017

CryoEM structure of SpMED at 4.4 Å and of SpMED-RNA pol II at 7.8 Å Tsai et al Nature 2017 –EMD 8479 –PDB 5U0P

Crystal structure of SpMED at 3.4 Å Nosawa et al Nature 2017 –PDB 5N9J

CryoEM structure of core ScMED-PIC (46 polypetides including TFIIH) at 5.8 Å Schilbach et al Nature 2017 –EMD 3850 –PDB 5OQM

2018

Crystal structure of human Mediator subunit MED23 (Tail subunit) at 2.8 Å Monté et al Nat Comms 2018 –PDB 6H02

2019

CryoEM of hMED at ~ 6 Å El Khattabi et al Cell 2019 –EMD 20393